00The microplate reader market was valued at US$ 534. Renilla/Firefly luciferase activities were measured twice. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. Cell viability was measured in percentages (%) as compared to untreated cells, which were assumed to be 100% viable. 8 Pages. Read Review (1)FlexStation® 3 Multi-Mode Microplate Reader. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. This microplate reader also features BioTek’s Hybrid Technology, which. 100 fmol 384-well (75 µL) 0. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 012 fmol 0. 5 ml 0. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 9. LDH cytotoxicity assaySome of new FilterMax F5 Multi-Mode Microplate readers are shipped with ARP USB to serial port adaptor (Manufacturer No: 572213, ARP No: 465338, 1 X USB, 1 X serial DB9/m, blue). Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. ** Some compatible filter sets are available for. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. The results are expressed as cell viabilities (%) based on the control condition. 7°C (gradient heating design). 9 mM H 2 O 2 for. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by. Instrument Configuration: Assay Technology Adapta® & • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate. Instrument Configuration: Assay Technology Adapta® & F3/F5 Multi-Mode Microplate Readers. GPCRs. The experiment was carried out with four biological replicates and two technical replicates. Datapaq Express (Vial Rack Bottom Label 2D Reader) XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING. FITC-conjugated casein assay substrate was added to the wells and incubated at room temperature for 2 hours. Products : General, FilterMax F5 reader. FilterMax F3 & F5 Microplate Readers. Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). By category. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535 nm, 595 nm, 625 nm; FP: 535 nm x 2), Excitation Filter Slide #2 (Absorbance: 260 nm, 340 nm, 405 nm, 450 nm, 595 nm, 620 nm), SoftMax Pro Microplate Data Acquisition and. MetaMorph NX. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. The OD 595 of the culture in the plate (light path length: ∼6. Click Run. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). Search LabX. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. FilterMax F5 Multi-Mode Microplate Reader. FilterMax F3 & F5 Microplate Readers. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). DCF fluorescence was measured by using a microplate reader (FilterMax F5) at maximum excitation and with emission spectra of 485 nm and 535 nm, respectively. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular devices, CA, USA). 4 Pages. Generally when this occurs, the destination BIG-IP device is unable to execute the tmsh command successfully. MetaFluor Software. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate Data Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceF3/F5 Multi-Mode Microplate Readers. 425 or. Page 24: Synergy™ Ht Multi-Mode Microplate Reader Internal plate shaking is also supported. , Sunnyvale, CA, USA). 4 fmol/100 μl fluorescein in Fluorescence Intensity-Top Read Mode, 0. State-of-the-art Multi-Mode Microplate Reader. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). Readers ®Examples: Monochromators such as the SpectraMax i3 Multi-Mode Microplate Reader Detection Platform or filter based systems like the FilterMax™ F3 and F5 Multi-Mode Microplate Readers Luminescence Principle The total amount of light emitted is collected Plate specs White plates to reflect all the light to the detectorSpectraMax i3/i3x Multi-Mode Microplate Reader HeLa cells Cell viability assay SoftMax Pro Software Simplified homogenous experimental protocol The EarlyTox cell viability assay kits apply a no-wash, homogeneous protocol that simplifies the workflow and reduces the data variability typically encountered with multiple experimental steps. The absorbance of the samples was determined using a FilterMax F3&F5 Multi-Mode Microplate Reader (Molecular Devices). HTRF-compatible SpectraMax Multi-Mode Readers. 4. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). used. book Page 1 Tuesday, September 7, 2010 6:14 PM. A microplate reader is laboratory machine used for the estimation of biochemical or. After adding 100 μl of new ice-cold PBS to the cells, fluorescent intensity was immediately determined using the fluorescent plate reader (FilterMax F3&F5 Multi-Mode Microplate Reader, Molecular Devices). Perception (Vial Rack Bottom Label 2D Reader) Microscan. SpectraMax Quant dsDNA Assay Kits. Supplier: Molecular Devices. Other technologies such as imaging. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. 561. Homogeneous Time-Resolved Fluorescence (HTRF) . Add to my manuals. Before unpacking and setting up the FilterMaxTM F3 or FilterMaxTM F5 Microplate Reader, prepare a dry, flat work area that has sufficient space for the instrument, host computer,. The absorbance values of the results were expressed as optical density (OD) as the mean ± standard deviation. 3 Pages. Luminescence (glow) Fluorescence polarization. – ™FilterMax F5 Multi-Mode Microplate Reader (cat. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. 00. Plate Formats:. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). Upgradeable modules are also available including western blot, cell imaging, and fast. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. 2 μM ATP standard was. Luminescence (glow) Fluorescence polarization. When the user applies a set temperature of 37°C, the top is heated to 37. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by normalizing to total protein. The absorption was measured in a spectrophotometer ultraviolet–visible (UV–Vis) Filter Max F5 multi-mode microplate reader; a wavelength of 595 nm was taken as the standard value for bacteria. The present study found competitive inhibition of hMAO-A by ILG along with a similar mode of action against hMAO-B as in rMAO-B. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. Neurotransmitter Transporter Uptake Assay Kit. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Cytokines Assay. 2 Pages. ” e. SpectraMax Glo Steady-Luc Reporter Assay Kit. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. MS-3 Laser Bar Code Reader. Multi-Mode Readers. SpectraMax Paradigm Multi-Mode Microplate Reader. The solution was incubated for 15 min and fluorescence was measured on a SpectraMax microplate reader (Molecular Devices). 3. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). 5. Absorbance microplate assays For more than 30 years, absorbance-based detection has been the preferred mode for many microplate-based assays, such as ELISA, protein, and nucleic acid quantitation or enzymatic assays. They provide absorbance, fluorescence, glow. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $ 3,250. Accurate measurements are ensured by automatic lamp calibration prior to each reading. Measurements of Caspase-3/7 Activity. Microplate Readers. A dual-color pyrometer with a selected wavelength away from that of the laser radiation has been used to monitor the melt pool temperature. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe parasite viability was determined by resazurin assay (0. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). After 24 h, the. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. 8 Pages. 0´ 104 1. 005 OD. 1 at 600 nm as determined by FilterMax F5 multi-mode microplate readers (Molecular Devices). Fred A. The following document is intended to demonstrate setup of this instrument. 3 Pages. Luminoskan luminometric microplate reader. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. SpectraMax Mini Multi-Mode Microplate Reader. F3/F5 Multi-Mode Microplate Readers. Many absorbance assays use a chromogenic substrate, which, upon enzymatic conversion to the final product, results in a compound thatThen 200 µl of 10% (v/v) ammonium hydroxide was added to neutralize the acid and absorbance readings were taken at 405 nm with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices. FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The increase in NADH, an end product in this reaction, was determined by means of its light absorbance at 355 nm using a fluorometric microplate reader (FilterMax F5 Multi-Mode Microplate Reader; Molecular Devices,. 4 Pages. Catalog. CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Other recommended products. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Instrument Setup: 1. The amount of the colorimetric product was monitored continuously for 120 min. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Instrument Configuration: Assay Technology Adapta® & Setup for GeneBLAzer® Assay on Molecular Devices FilterMax™ F5 Microplate Reader with SoftMax® Pro 6. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance. Delete from my manuals. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. SpectraMax Paradigm Multi-Mode Microplate Detection System from Molecular Devices, LLC. Supernatant (25 μL), or standard, was added and mixed. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. DMSO was used to dissolve the compounds and was included in the microplate at 0. SpectraMax Paradigm Multi-Mode Microplate Reader. C1–F5 Filter Set: EX 360/40 nm EM 460/40 nm Optics Position: Gain: Auto Scale to High Wells Scale Well: C1 Scale Value: 30000 Delay after Plate Movement: 100 msec Synergy. FlexStation 3 Multi-Mode Microplate Reader. Yes, the M5 does have a limited slip. 2. Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 4. We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC. Protein contamination can be measured at 280 nm. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 6 Pages. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). F3/F5 Multi-Mode Microplate ReadersCell pellets were lysed and HTLV-1 LTR-transactivation was measured via luciferase assay according to the manufacturer’s protocol (Dual-Luciferase ® Reporter Assay System, Promega Corporation, Madison, WI; Filtermax F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) . 3 Pages. Absorbance at 570 nm was recorded on a CLARIOstar multi-mode microplate reader (BMG Labtech, Germany). (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. 1 at 600 nm as determined using a FilterMax F5 multi-mode microplate reader (Molecular Devices). 6. Cells were seeded in 24-well plates and cultured. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. 4. --> Life sciences. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. Molecular Devices’ FilterMaxTM F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter incubation for 6 minutes, the mixture was read at 340 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Readers, Molecular Devices). Vascular. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. The plates were loaded into a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA), and the fluorescence intensity was measured at 37 °C with Ex/Em = 440/484 nm and 15 s shaking between reads to facilitate aggregation. Read Review (1)With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Instrument Configuration: Assay Technology Adapta® & FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell-based application requirements while. In addition, the FilterMax F5 Multi-Mode Microplate Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. 4 Pages. LabX is a multi-vendor marketplace that connects you with the life science tools and chemistry you need to run your lab. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. 2 Pages. FilterMax F5 Multi-Mode Microplate Reader. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. F3/F5 Multi-Mode Microplate Readers The FilterMax Series of microplate readers includes: FilterMax F3 Multi-Mode Microplate Reader. 00 SpectraMax M3, M4, M5, and M5e Multi-Mode Document type: User Manual5016208D 8 Chapter1:Introduction TheFilterMax™ F3Multi-ModeMicroplateReaderandtheFilterMax™F5Multi-Mode MicroplateReaderareidealforabroadrangeofapplications. 7°C and the bottom to 36. FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#:. SpectraMax MiniMax 300 Imaging Cytometer for the SpectraMax i3x Multi-Mode Microplate Detection Platform KEY FEATURES • ombine cellular imaging C and microplate assays into a single workflow • liminate cell staining for cell E counting and confluency with StainFree Technology • omplete cell viability C and toxicity assays with. Fluorescence was measured with excitation and emission wavelengths of 530 and 590 nm, respectively, on a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Caspase-3 Activity. shopping cart 0 items $0. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. For customers outside the U. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. All experiments were performed in triplicate. Open SoftMax® Pro software. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. In the list below the slide number 21, select the positions where the emission filters are installedF3/F5 Multi-Mode Microplate Readers. 0. Related Products: FilterMax F3 &. Transfluor Technology. SpectraMax Quant dsDNA Assay Kits. Approximately 50 µl of these. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. CloneMatrix. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. aureus , the amount of bacteriophage-MBs and the dilution ratio of the HRP. Related Products: FilterMax F3 & F5 Multi-Mode Microplate Readers, SpectraMax i3 Multi-Mode Platform Microplate Detection System,. Zebrafish whole-eye mRNA extraction and RT-PCRSearch LabX. A) CHO colonies, serum-free suspension-adapted, in CloneMedia-CHO. 3 and higher include optimized reader settings, as well as data analysis, for faster results. Owner, Zeckhausen. Absorbance mode reads plates up to 0. Mettler Jr. Absorbance Detection Comparison Table. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 00 USD. 00. x phosphorylation of wild-type BY4741 yeast exposed to 1 μg/ml fludioxonil in 1% DMSO or 0. These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes (typically absorbance, fluorescence and luminescence). Click to Contact Seller. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. 00. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. 561. 1 (GraphPad Software Inc. The readings of samples incubated with different carbon sources were subtracted from the average of the control readings. The supernatant was taken and 0. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. GPCRs. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. A microplate reader (also called sometimes microtiter plate reader) is a high-sensitivity instrument designed to analyze samples contained in microplate wells. The standard curve presented a linear phase from 1. 0. Then, 50 µL of a stop solution were applied to terminate the reaction. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 3 Pages. 50/50. They provide absorbance, fluorescence, glow luminescence, and other read modes for Affordability and unmatched assay performance in filter-based microplate readers. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. SpectraMax iD5. 6% from 2020 to 2027. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. Instrument Configuration: Assay Technology Adapta® & Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET only) (TR-FRET only) HTRF Certification LanthaScreen Certification Key. The following document is. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. ScanLater Western Blot Detection System. Detection cartridge modularity allows for easy addition of new read modes within minutes without. FilterMax F3 & F5 Multi-Mode Microplate Readers. Samples (180 μl) were stained with 20 μl of Nile red (2. Ultrafast Multi-Elemental Analysis Atomic Spectroscopy Consumables & Accessories. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. The inter-assay coefficient of variations (CV) and values for all biomarkers can be found in Table 4. FilterMax® F3 & F5 Multi-Mode Microplate Readers. KG (CAT#: STEM-LE-1659-Y)The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAfter 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). Absorbance was measured at 450 nm using a FilterMAx F5 Multi-Mode Microplate Reader and EX2 filter after a 2-hour incubation at 37°C with 10 μL of CCK-8 per 100 μL of media. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. 3 Pages. Please note it is NOT a Keyspan USA-19 HS USB adaptor that is commonly used by. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 425 or. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. 00FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. microplate reader. 100 fmol 1536-well (8 µL) 0. Click “Eject Plate Carrier. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. ImageXpress Micro 4 High-Content Imaging System. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. 4. 2. Capabilities: FilterMax F5 Multi-mode microplate reader. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. This instrument has passed specific verification criteria. An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. GenePix 4300/4400 Microarray Scanner. Description: The FilterMax™ F3 features absorbance (visible),. If using a multi-functional reader, please be sure to check the sensitivity of the device using the MycoAlert™ Assay Control Set. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. Luminescence was recorded with a luminometer (Filter Max F5 Multi-Mode Microplate reader) with SoftMax Pro Easy software, and the NADH/NAD + ratio was calculated by comparing the relative light units. MetaXpress. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell. Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. CatchPoint cGMP Detection Assay Kit. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solu. The haemolytic activity was determined in the cell supernatant by optical density readings at 570 nm (FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices). 003 fmol 0. The Labsystems Nepheloskan Ascent Microplate Reader is designed to run with 96-well microplates, for use on applications like immunoprecipitation reactions as well as solubility tests of pharmaceutical components. Time-resolved Fluorescence. SpectraMax iD5. 0. The multi-mode microplate reader provides flexibility and ease of use for a variety of applications Market Highlights Microplate Readers Market is predicted to project a notable CAGR of 4. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. Add to my manuals. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The amount of colorimetric product was continuously monitored for 120 min. 3 Pages. 3 Pages. USD ($) USD - United States Dollar. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. Each kit was used on the same day that its reagents were reconstituted. Chemiluminescence measurements were performed using a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). 3. CloneMatrix. 1% (v/v) formic acid, was adopted for lipid separation: 0–2 min. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $3,500. DHE fluorescence was recorded on a Filtermax F5 multi-mode microplate reader. The 0. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. Microplate Reader Comparison Charts. Load prepared PicoGreen plate (step C. SpectraMax Glo Steady-Luc Reporter Assay Kit. FilterMax F5 Multi-mode microplate reader. Upgradeable modules are also available including western blot, cell imaging, and fast. 00. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). Fluorescence of the organic phase was measured at 515 nm of excitation and 553-nm emission using a fluorimeter (FilterMax F5-Multi-Mode Microplate Reader, Molecular Devices, CA, USA). Ease of use. 3 Pages. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. MetaMorph NX. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. Match case Limit results 1 per page. Configurability is an important aspect of BioTek’s multi-mode readers to provide the most value for laboratory budgets, but the independent optical systems in our multi-mode readers don’t compromise on performance. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. Devices FilterMax Multi-Mode Microplate Readers. Molecular Devices Microplate reader VersaMax $1,495. 8 Pages. A Vision for the Future With TTP LabTech. Biofilm quantification and scanning electron microscopy (SEM) analysis. In addition, the instrument features linear and orbital shaking, plus temperature. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . For more detailed information and technical support of Life Technologies assays, please call 1-800-955-The next day, precultures were diluted to an optical density of 0. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. The measurement can be performed using a variety of detection technologies: the. 97059-524EA 84727. Each condition was performed in. A blank sample was prepared by mixing. 00. Not for use in diagnostic procedures. 020 fmol 0. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. $3,000 USD. Each condition was performed in. Activation of TLR4 and TLR2 receptors. A microplate consists of small wells wherein separate reactions take place.